Synergistic solid-state/liquid approach

To successfully tackle membrane proteins and the conversion of monomeric protein to large macromolecular complexes researchers need often both techniques and therefore access to both the liquid state NMR facility in Gothenburg and the solid-state NMR facility in Umeå.

At the Swedish NMR Centre there is not only support in producing sufficient amount of membrane proteins for Swedish researchers but also to study smaller membrane proteins (1-2 TM) in micelles by liquid NMR to provide some initial structural information prior to further refinement by SS NMR using proteoliposomes. For larger proteins and especially transport systems, the main work will be by SS NMR since these systems are only functional and fully active in the correct membrane environment (e.g. transport activities or signal transduction not possible in micelle systems). Nevertheless preliminary work by high-field solution NMR in combination with specific labeling concepts is often necessary initially (e.g. assignment strategies and determination of correct conformations etc). In addition, solution NMR approaches like saturation transfer difference NMR are extremely valuable for systems where membrane proteins are used as targets in ligand and small molecule drug screening. Aggregating proteins induce neurodegenerative diseases and the whole process of aggregation from the soluble form via the oligomeric form to the fibril needs to be investigated, preferably by a combination of liquid and solid state NMR; available by our NMR initiative here. Our joint initiative maximizes the innovative potential and places combined synergistic solid-state/liquid NMR conducted in Sweden at the forefront of international structural biology research.

Contact

Jürgen Schleucher
professor, Co-director, Swedish NMR Centre Umeå
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