Cell-free protein expression

Cutting edge NMR optimized cell-free protein expression

The need for advanced protein production technology has become instrumental for contemporary NMR analysis in large part due to the increasingly complex protein systems under study. Cell-free protein synthesis technology has emerged since it allows control of complex substrates beyond what is available in traditional cell-based systems, simply due to the inherent metabolic activity associated with in vivo protein production. A system for cell-free protein synthesis in batch mode for NMR purposes has been developed at the Gothenburg node of the Swedish NMR Centre. This system has been used for successful production of all classes of proteins. CFPS is available for users of the infrastructure to enable efficient structure determination of biological macromolecules.